The Silent Guardian in the Grain Silo

How CO2 Monitoring Is Revolutionizing Food Safety

A simple gas sensor is now the most powerful weapon against invisible grain contaminants.

Imagine a world where millions of tons of food become contaminated before ever reaching a plate. This isn't a hypothetical scenario—it's a persistent global challenge in grain storage. Now, scientists have discovered an unlikely ally in this battle: carbon dioxide. In storage silos worldwide, real-time CO2 monitoring is emerging as an early-warning system against grain spoilage and dangerous mycotoxin contamination, offering a powerful tool to safeguard our food supply and reduce waste.

The Unseen Threat in Our Grains

Mycotoxins are toxic compounds produced by moulds that commonly grow on staple cereals like wheat, maize, and oats. These heat-stable molecules are difficult to remove or inactivate once formed in a food product. Exposure through consumption can lead to serious health issues including immunotoxicity, teratogenicity, mutagenicity, and carcinogenicity. Their presence has prompted strict international regulations, particularly in the European Union/UK where maximum legal limits exist for aflatoxins, ochratoxin A, deoxynivalenol, and other mycotoxins in cereals2 .

Global Grain Loss

The post-harvest phase represents a critical vulnerability point in our food system. Poor storage management can result in significant quality losses due to either pest activity or mould spoilage with associated mycotoxin contamination.

Grain Loss Statistics

5-10%

Developed Countries

30-35%

Lower Middle-Income Countries

Estimated grain loss percentages in different economic contexts2

Why Carbon Dioxide Holds the Key

All living grains respire, naturally producing CO2 as they break down stored carbohydrates. However, when moulds and fungi begin to grow, this respiration increases dramatically. Elevated CO2 levels thus serve as an early indicator of biological activity from spoilage microorganisms long before visible damage or temperature changes occur2 7 .

Traditional Temperature Monitoring

Grain's insulating properties mean temperature changes often lag significantly behind actual spoilage. By the time a temperature cable detects a "hotspot," significant damage may have already occurred.

CO2 Monitoring Advantage

As one industry expert notes, "CO₂ levels move more rapidly through air currents than heat conducts through grain"4 . This makes CO2 monitoring a faster and more sensitive approach to spoilage detection.

A Closer Look: The Wet Pocket Experiment

A groundbreaking study published in the Journal of the Science of Food and Agriculture provides compelling evidence for CO2 monitoring's effectiveness. Researchers designed a comprehensive experiment to compare real-time CO2, temperature, and relative humidity sensors as indicators of stored grain quality1 2 .

Initial Laboratory Experiments

Used mini-silos containing naturally contaminated wheat grain stored at different moisture contents (15-30%) to evaluate effects on grain respiration1 .

Large-Scale Study

Utilized two pilot-scale silos holding 2.5 tonnes of wheat each, equipped with ATEX-compliant CO2/RH/T sensors. To simulate a real-world storage problem, researchers created a 'wet pocket' by introducing water to a localized area, mimicking the water ingress that might occur through a leak or condensation1 2 .

Revealing Results: CO2 Outperforms Traditional Methods

The findings were striking. The simulated wet pocket triggered a rapid rise in CO2 levels while temperature remained relatively stable1 . This demonstrated CO2's superior sensitivity as an early warning indicator.

Comparison of CO2 and temperature responses to spoilage conditions

Mycotoxin Analysis

The affected wet pocket area showed a clear increase in the concentration and diversity of mycotoxins, particularly aflatoxin B1, aflatoxin B2, deoxynivalenol, deoxynivaenol-3-glucoside, and moniliformin1 . The unaffected regions maintained significantly lower contamination levels.

Mycotoxin Concentration in Affected vs. Unaffected Grain Regions

Mycotoxin Affected Region Unaffected Region
Aflatoxin B1 Significantly Elevated Lower
Aflatoxin B2 Significantly Elevated Lower
Deoxynivalenol Significantly Elevated Lower
Deoxynivalenol-3-glucoside Significantly Elevated Lower
Moniliformin Significantly Elevated Lower

Table 1: Comparison of mycotoxin levels in grain samples from affected and unaffected regions of storage silos1

Respiration Rates in Oats Under Different Storage Conditions

Water Activity (a_w) Temperature Respiration Rate (μg CO₂ kg⁻¹ h⁻¹)
0.70 15°C Low
0.70 20°C Low
0.90 15°C Moderate
0.90 20°C Moderate
0.95 15°C High (≥25)
0.95 20°C High (≥25)

Table 2: Respiration rates increase with higher water activity and temperature, with mycotoxins exceeding legal limits when respiration rates reached ≥25 μg CO₂ kg⁻¹ h⁻¹ at water activity levels ≥0.906

The Scientist's Toolkit: Essential Research Equipment

Implementing an effective CO2 monitoring system requires specific equipment and technologies. Here are the key components used in the featured research:

ATEX-compliant CO₂/RH/T sensors

Measures real-time gas concentrations, humidity, and temperature. Deployed in pilot-scale silos for safe, continuous monitoring1 2 .

Infrared CO₂/T sensor

Precisely detects CO₂ concentrations and temperature. Used in mini-silo experiments (0–15% ±0.2% accuracy)2 .

Mini-silo experimental setup

Small-scale controlled environment for initial testing. Contained naturally contaminated grain at varying moisture levels2 .

Pilot-scale silos

Intermediate-sized storage systems for realistic trials. Held 2.5 tonnes of grain to bridge lab findings with real-world conditions1 .

Water activity meter

Measures available water in grain samples. Confirmed moisture content and water activity levels in grain samples2 .

LC-MS/MS technology

Identifies and quantifies multiple mycotoxins. Analyzed grain samples for precise mycotoxin contamination levels6 .

Table 3: Essential research equipment for CO2 monitoring studies in grain storage research

From Research to Real-World Application

The implications of this research extend far beyond laboratory settings. Companies are already developing commercial applications based on these principles. As one industry provider explains: "Carbon dioxide monitoring provides a real-time indicator of grain spoilage by detecting changes in CO2 levels, which move more rapidly through air currents than heat conducts through grain"4 .

Practical Installation

Practical installation typically involves placing CO2 sensors in either the headspace above the grain or the plenum below it, connected to monitoring nodes that transmit data for analysis4 .

Headspace Monitoring Plenum Monitoring

Integration Benefits

This technology integrates with existing temperature cable systems, significantly enhancing grain storage management without requiring complete infrastructure overhaul.

  • Reduces need for blanket fumigation
  • Minimizes unnecessary grain movement
  • Lowers economic costs and environmental impact
  • Maintains grain quality throughout storage

The Future of Grain Storage

Research continues to refine our understanding of the relationship between CO2 levels and grain quality. Recent studies have examined how different grains produce varying CO2 signatures under comparable conditions, potentially allowing for grain-specific monitoring protocols6 .

Predictive Modeling

The integration of predictive modelling with real-time CO2 data represents another frontier, potentially enabling silo managers to anticipate contamination risks before they materialize2 .

Climate Change Impact

As climate change alters storage conditions and increases contamination risks in major grain-producing regions, the importance of effective monitoring technologies becomes even more pronounced2 .

A More Resilient Food System

What begins as a simple CO2 sensor in a silo ultimately contributes to a more resilient, efficient, and safe global food system—proof that sometimes the most powerful solutions emerge from watching the invisible signs nature provides.

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